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Fig. 5 | Journal of Cannabis Research

Fig. 5

From: Evaluation of the metabolic activity, angiogenic impacts, and GSK-3β signaling of the synthetic cannabinoid MMB-2201 on human cerebral microvascular endothelial cells

Fig. 5

The formation of angiogenic tubular structures was induced in endothelial cells after treatment with the synthetic cannabinoid MMB-2201. A total of 20,000 HBEC-5i cells were seeded in a pre-cooled 96-well plate coated with basement membrane extract. (A) Microscopic images captured 24 h after treatment with MMB-2201 illustrate the development of tubular structures. A quantitative analysis of the angiogenic parameters was conducted, including (B) total tube length, (C) the number of tubular structures, (D) the number of loops, and (E) the number of branches. A significant increase was observed at concentrations of 0.01 µM and 1 µM of MMB-2201. Data are presented as mean ± SEM (n = 3). (****) indicates p < 0.0001

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Journal of Cannabis Research

ISSN: 2522-5782