Fig. 3
The synthetic cannabinoid MMB-2201 increased the metabolic activity in endothelial cells. A total of 5000 HBEC-5i cells were counted and seeded in a 96-well plate for 24 h. Different concentrations of MMB-2201 were then incubated with the cells for 24 h. Subsequently, the media containing MMB-2201 was discarded, and the cells were treated with MTT solution and incubated for 4 h at 37 °C and 5% CO2. The formazan crystals were then solubilized with DMSO, and absorbance was measured. The treatment significantly enhanced cell viability at concentrations ranging from 0.001 µM to 1 µM compared to the control. Data are presented as mean ± SEM (n = 3). (*) indicates p < 0.05