Fig. 8

Infiltration of CD4⁺ T cells into the spinal cord of cannabis-treated mice. MOG35-55-given female C57BL/6 mice were treated with vehicle, CN2, or CN4. Spinal cords were removed and cut horizontally. Sections were stained using antibodies against CD4⁺ T cells (gray), and the cell nuclei were stained with DAPI (blue) and examined using a confocal microscope (X20 magnification). The images (A-H) are representative images of 2 independent experiments, with 5 mice in each group. In I, the graphical result is presented as the mean ±SEM of the color intensity to the area, n ≥10. *** - p <0.001 vs. control, ^^^p <0.001 vs. MOG, ### p <0.001 vs. CN4