Fig. 6

Microglial activation in the spinal cord of cannabis-treated mice. Female C57BL / 6 mice treated with MOG35-55 were treated with vehicle, CN2, or CN4. Spinal cords were removed and cut horizontally. The sections were stained by immunohistochemistry using antibodies against activated microglia (green), and the cell nuclei were stained by DAPI (blue) and examined using a confocal microscope (X20 magnification). The images (A-H) are representative images of 2 independent experiments, with 5 mice in each treatment group. In I, the presented results are mean ±SEM of the color intensity to the area of the Iba1 results (B,D,F,H), n ≥ 20 *** - p <0.001 vs. control, * p <0.05 vs. control , ^^ p <0.01 vs. MOG, ### p <0.001 vs. CN4