Fig. 2

Extracts of the cannabis cultivars CN2, CN4, and CN6 lowered iNOS protein expression in LPS-activated BV2 cells. BV2 cells were incubated for 24 h with LPS (7 ng/ml) and extracts from selected cannabis cultivars (20 µg/ml). At the end of the experiment, SDS PAGE and Western blot analysis were performed using antibodies against iNOS and β-actin. In A, the bands represent two independent experiments. In B, the results are mean±SEM of all experiments, (n=6). Statistical significance was determined by one-way ANOVA and Tukey-Kramer Multiple Comparison Post Test. *** - p <0.001 vs. control, ### - p <0.001 vs. LPS (n=6). *** - p <0.001 vs. control, ### - p <0.001 vs. LPS