Fig. 5

GRP78 knockdown in Panc03.27 cells does not affect the ATF/CHOP arm of the UPR pathway. (a) and (b) qPCR for GRP78 mRNA following transfection with 5 nM siGRP78 for 24 and 48 h. (c) Western blot (s) of housekeeping gene, alpha-tubulin, GRP78, ATF4 and CHOP following siRNA treatment and below plot represents ATF4 following 48 h treatment with cannabidiol at 10, 20 and 40 µM in Panc03.27 cells