Fig. 4

Ceramide synthase 1 knockdown reduces protein expression of GRP78 in Panc03.27 cells. (a) and (b) qPCR for CerS1 mRNA following transfection with 5 nM si-CERS1 for 24 and 48 h. (c) Bar blot of protein concentration (ng/ml) of CerS1 using ELISA following transfection with 5 nM si-CERS1 for 48, 72 and 96 h. (d) Bar blot of protein concentration (ng/ml) of CerS1 using ELISA following 48 h treatment with cannabidiol at 10, 20 and 40 µM. (e) Western blot (s) of housekeeping gene, alpha-tubulin, CerS1, GRP78 and CHOP following siRNA treatment. (f) Western blot (s) of GRP78, CHOP and alpha-tubulin following 48 h treatment with cannabidiol at 10, 20 and 40 µM