Fig. 2

Ceramide Synthase isoform 1 is upregulated by cannabidiol in pancreatic cancer. (a) Summary plot of mean and error with SD of CerS1 fold changes across all treatment groups of concentraions reflected in table 1’s ic50 values at 48 h, comparing human Panc03.27, Panc1 and murine-3275 cells. The subsequent statements indicate greatest to lowest significance of CerS1 upregulation with gemcitabine, abraxane and CBD (GAC) treatment in Panc03.27 cells followed by CBD treatment, then combination of gemcitabine and CBD (GC) and finally gemcitabine single treatment. In Panc1 cells, GAC treatment followed CBD single treatment and finally gemcitabine and abraxane (GA). Murine 3275 cells showed signficance in CerS1 upregulation in GC combination treatment only in comparison to control. (b) Using publicly available RNA sequencing data (GSEA, gene set enrichment analysis) of PDAC tumours versus normal, a Kaplan-Meier curve indicates higher CERS1 correlates with better overall survival (OS) (HR: hazard ratio = 0.61, Logrank p = 0.018, p = 0.019). Gem = gemcitabine, Abx = abraxane, CBD = cannabidiol