Table 4 Inter-day accuracy and precision for cannabinoids of the assay method. The results verify the reproducibility of the assay method as required by AOAC SMRP 2019.003 (Standard Method Performance Requirements (SMPRs) for Quantification of cannabinoids in plant materials of hemp (Low THC Varieties Cannabis sp.) 2019)
Concentration (ng/mL) | Cannabinoid | ||||||||
CBC | CBD | CBDA | |||||||
Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | |
10 | 10.3 ± 0.4 | 4.1 | 3.2 | 10.2 ± 0.4 | 4.0 | 1.8 | 10.4 ± 0.6 | 5.3 | 4.1 |
75 | 72.5 ± 4.6 | 6.4 | −3.3 | 72.5 ± 2.4 | 3.3 | −3.3 | 76.3 ± 4.7 | 6.1 | 1.7 |
750 | 760.1 ± 28.1 | 3.7 | 1.3 | 749.9 ± 21.4 | 2.9 | 0.0 | 739.0 ± 30.5 | 4.1 | −1.5 |
1750 | 1868.4 ± 58.4 | 3.1 | 6.8 | 1841.3 ± 48.9 | 2.7 | 5.2 | 1734.9 ± 76.7 | 4.4 | −0.9 |
Concentration (ng/mL) | Cannabinoid | ||||||||
CBDV | CBG | CBGA | |||||||
Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | |
10 | 9.6 ± 0.6 | 6.0 | −3.8 | 10.3 ± 0.8 | 8.1 | 2.7 | 10.6 ± 0.9 | 8.2 | 5.7 |
75 | 73.6 ± 3.6 | 4.8 | −1.8 | 70.5 ± 3.7 | 5.3 | −-6.0 | 74.2 ± 4.2 | 5.7 | −1.0 |
750 | 765.8 ± 27.8 | 3.6 | 2.1 | 755.6 ± 24.9 | 3.3 | 0.7 | 735.0 ± 19.8 | 2.7 | −2.0 |
1750 | 1852.4 ± 56.3 | 3.0 | 5.9 | 1880.0 ± 58.9 | 3.1 | 7.4 | 1731.0 ± 77.0 | 4.4 | −1.1 |
Concentration (ng/mL) | Cannabinoid | ||||||||
CBL | CBN | Δ-8-THC | |||||||
Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | |
10 | 10.3 ± 0.5 | 4.4 | 3.0 | 9.8 ± 0.4 | 4.3 | −1.8 | 10.3 ± 0.4 | 4.3 | 3.3 |
75 | 74.5 ± 4.6 | 6.2 | -0.7 | 76.8 ± 4.0 | 5.1 | 2.4 | 75.4 ± 5.0 | 6.7 | 0.5 |
750 | 779.6 ± 25.6 | 3.3 | 4.0 | 755.9 ± 29.2 | 3.9 | 0.8 | 778.8 ± 35.5 | 4.6 | 3.8 |
1750 | 1877.3 ± 67.3 | 3.6 | 7.3 | 1736.0 ± 55.0 | 3.2 | −0.8 | 1762.2 ± 52.5 | 3.0 | 0.7 |
Concentration (ng/mL) | Cannabinoid | ||||||||
Δ-9-THC | THCA | THCV | |||||||
Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | Measured Concentration (mean ± SD) | Precision (%RSD) | Accuracy (% Bias) | |
10 | 9.8 ± 0.6 | 5.9 | −1.6 | 9.8 ± 0.7 | 6.8 | −1.9 | 10.0 ± 0.4 | 4.4 | 0.0 |
75 | 77.9 ± 3.3 | 4.2 | 3.8 | 79.0 ± 4.1 | 5.2 | 5.3 | 78.3 ± 3.4 | 4.3 | 4.3 |
750 | 758.2 ± 24.8 | 3.3 | 1.1 | 719.7 ± 34.1 | 4.7 | −4.0 | 760.4 ± 33.1 | 4.4 | 1.4 |
1750 | 1741.8 ± 64.9 | 3.7 | −0.5 | 1621.6 ± 100.2 | 6.2 | −7.3 | 1738.6 ± 59.1 | 3.4 | −0.6 |